Bishu Roy was born into a musical family in a village in West Bengal. Pandit Roy started his musical journey at an early age with flute under the tutelage of Sri Birendra Nath Bhattacharya and subsequently under Guru Gour Goswami. There he was introduced to the Maihar Gharana style of Hindustani music. Here he adopted the sitar-sarod style of playing with long alap. jor and jhala. Later in life, Pandit Roy trained under Guru Ajoy Sinha Roy.